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一个实用的工作流程,用于固定和减少死后人类大脑组织的相关光和电子显微镜的自发光

Ha-Eon Lee¹, Hong Lim Kim², Ara Cho³

¹Department of Anatomy, Catholic Neuroscience Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Medical Science, College of Medicine, Graduate School of The Catholic University of Korea, Seoul, Republic of Korea.

|December 24, 2025

概括

这项研究优化了一种工作流程,用于使用相对光和电子显微镜 (CLEM) 分析死后的人类大脑组织. 新方法提高了超结构的保存和抗原性,提高了未来神经病理研究的大脑样本的实用性.

科学领域:

神经科学是一个神经科学.
显微镜的使用方法
生物技术是生物技术.

背景情况:

通过CLEM进行死后人类大脑组织分析,面临着贫困的超结构和高自发光度的挑战.
现有的协议往往缺乏灵活性用于脑储存和各种未来研究.

研究的目的:

建立一个有效和灵活的保存和处理工作流程,用于对死后人类大脑组织进行CLEM分析.

主要方法:

评估的固定协议 (4% PFA与4% PFA + 0.2% GLA) 和自光火器.
使用共聚焦和传输电子显微镜 (TEM) 评估免疫光强度和超结构完整性.

主要成果:

用4%的PFA + 0.2%的GLA固定改善了超结构,而不会影响免疫活性.
自体光火器降解了超结构,使它们不适合电子显微镜.
成功执行了高保真性CLEM,将免疫标记结构与超结构关联起来,并识别了脂素颗粒.

结论:

为死后的人类大脑组织建立了平衡超结构保护和抗原性的自适应工作流.
用4%的PFA + 0.2%的GLA固定,然后冷保存,可以创建一个灵活的,高质量的组织档案.
省略基于溶剂的火器对于高准确度的CLEM和电子显微镜至关重要,提高了人类大脑样本的实用性.

关键词:

自动光是一种自发光.相对光和电子显微镜的相对光和电子显微镜.电子显微镜的电子显微镜固定方式固定方式死亡后的人类大脑.

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