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MMonitor用于实时监测微生物群落,使用长读数

Timo N Lucas¹, Ulrike Biehain², Anupam Gautam³

¹Institute for Biomedical Informatics (IBMI), University of Tübingen, Sand 14, 72076 Tübingen, Germany; Cluster of Excellence "Controlling Microbes to Fight Infections" (CMFI), University of Tübingen, Auf der Morgenstelle 28, 72074 Tübingen, Germany.

Cell reports methods

|December 24, 2025

概括

MMonitor是一个新的开源软件,用于对牛津纳米孔技术 (ONT) 测序数据的实时元基因组分析. 它可以轻松监测微生物群落,并生成高质量的元基因组组装基因组 (MAG).

科学领域:

微生物学微生物学
生物信息学是一种生物信息学.
计算生物学计算生物学

背景情况:

微生物群落的实时监测提供了对环境动态的关键见解.
现有的元基因组分析工具往往缺乏用户友好性和实时监控功能,需要大量的计算专业知识.

研究的目的:

介绍MMonitor,一个开源软件平台,用于实时元基因组数据分析和可视化.
通过牛津纳米孔技术 (ONT) 测序数据,提供一个用户友好的解决方案来监测微生物群落.

主要方法:

MMonitor包括一个桌面应用程序,GUI/CLI选项和一个基于Web的仪表板,用于交互式数据探索.
综合管道促进了自动化de novo组装和重建元基因组组装基因组 (MAG).
验证涉及跟踪人类肠道微生物种群和全基因组测序 (WGS) 数据分析.

主要成果:

MMonitor仪表板可视化了分类学组成,质量评分,多样性指数以及随时间推移的分类学-元数据相关性.
高质量的注释MAG是由WGS数据生成的.
MMonitor与现有的实时元基因组工具进行了比较,强调了其优势和局限性.

结论:

MMonitor为实时元基因组分析提供了一个强大且易于使用的平台,特别是用于ONT测序数据.
该软件促进了有效的微生物社区监测和MAG重建,帮助各种研究应用.

关键词:

CP:生物技术生物技术培训班:微生物学生物信息学软件生物信息学软件生物技术是生物技术.数据可视化数据可视化微生物组是一个微生物组.纳米孔测序的测序实时元基因组学实时元基因组学

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