在葡萄酒发酵过程中与Saccharomyces cerevisiae和Oenococcus oeni进行分阶段解决的转录调节和代谢相互作用
Qing Sun1,2, Xinghui Pan2,3,4, Yao Lu1
1College of Enology, Northwest A&F University, Yangling, Xianyang, Shaanxi 712100, China.
Journal of agricultural and food chemistry
|December 24, 2025
概括
这项研究揭示了Oenococcus oeni如何在葡萄酒发酵过程中影响Saccharomyces cerevisiae,影响风味和微生物相互作用. 了解这些动态有助于设计更好的葡萄酒发酵工艺,以提高品质.
科学领域:
- 微生物学 微生物学
- 发酵科学 发酵科学
- 代谢工程是代谢工程.
背景情况:
- 葡萄酒的风味和发酵涉及复杂的微生物相互作用,主要是Saccharomyces cerevisiae和Oenococcus oeni之间.
- 之前的研究表明,O. oeni的结会影响葡萄酒的酸性和香味特征,但根本的分子机制和代谢策略尚未完全理解.
研究的目的:
- 为了阐明S. cerevisiae和O. oeni在混合发酵过程中的转录和代谢相互作用.
- 了解O. oeni如何影响S. cerevisiae的生长动力学和基因表达.
- 揭示控制混合葡萄酒发酵的代谢策略和相互作用.
主要方法:
- 进行了特定阶段的S. cerevisiae和O. oeni.混合发酵.
- 利用时间解析的转录学来分析基因表达动态.
- 集成了一个社区基因组规模的代谢模型与转录组数据进行流量分析.
主要成果:
- O. oeni改变了葡萄酒的酸性特征和芳香复杂性,影响了S. cerevisiae的生长.
- O. oeni调节了S. cerevisiae的转录反应,特别是在压力和硫代谢基因中.
- O. oeni通过基酶途径利用果糖,影响酸盐的形成,并与S. cerevisiae进行氨基酸和曼尼托尔的交换.
结论:
- 这项研究提供了关于S. cerevisiae-O.O.的全面观点. 在混合发酵过程中发生的任何相互作用.
- 获得了对微生物合作和竞争的机制性见解.
- 这些发现为葡萄酒发酵的合理设计提供了策略,以提高风味质量.
相关概念视频
01:17Gene Regulation During Sporulation
401
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
401
01:23Microbial Fermentation
1.3K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.3K
01:23Transcriptional Regulation: Riboswitches
514
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
514
01:28Gene Regulation in Microbial Communities: Quorum Sensing
457
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
457
01:28Yeast Signaling
17.0K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.0K
01:19Regulation of Metabolism
11.3K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.3K