青素-杆菌素治疗重新连接HepG2细胞的核心代谢和核糖体程序
Cameron S Movassaghi1,2,3, Jesse G Meyer1,2,3
1Department of Computational Biomedicine, Cedars Sinai Medical Center, Los Angeles California 90048, United States.
Journal of proteome research
|December 24, 2025
概括
在细胞培养中使用抗生素会影响蛋白质水平,特别是在核糖体和线粒体蛋白质中. 这些蛋白质变化最初最显著,并在通道上稳定.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 细胞生物学 细胞生物学
- 哺乳动物细胞培养
背景情况:
- 抗生素在细胞培养中很常见,但可能会影响实验结果.
- 已知抗生素的基因组和转录组效应,但蛋白质组影响的理解较少.
研究的目的:
- 综合分析培养有或没有抗生素 (青素/链杆菌素) 的 HepG2 细胞中的全蛋白质组差异.
- 研究抗生素治疗对哺乳动物细胞蛋白质的时间效应和保存影响.
主要方法:
- 纵向和交叉研究设计,采用119个样本,跨越9个通道和4个条件.
- 使用质谱法检测和量化蛋白质的蛋白质组分析.
- 线性混合效应建模以评估时间变化和治疗启动/停止效应.
主要成果:
- 在接受抗生素治疗和未接受治疗的疾病之间,确定了383种不同丰富的蛋白质.
- 观察到核糖体和线粒体蛋白质的显著变化,表明非目标效应.
- 蛋白质影响在第一个通道最强,在第三个通道稳定.
结论:
- 哺乳动物细胞培养中的抗生素治疗诱导了显著的,可测量的蛋白质变化.
- 引入或移除抗生素的时间影响了蛋白质基因变化的程度.
- 这些发现为了解保护抗生素效应和设计未来细胞培养实验提供了基础.
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