相关实验视频
Updated: Jan 7, 2026

07:39
Mouse In Vivo Placental Targeted CRISPR Manipulation
Published on: April 14, 2023
3.5K
miR-155在胎盘形态发生过程中抑制 angiotensin II 1 型受体合成
Anya L Arthurs1,2, Eugenie R Lumbers3,4, Lachlan Schofield3,4
1Flinders University, College of Medicine and Public Health, Flinders Health and Medical Research Institute, Adelaide, SA, Australia.
Cell death discovery
|December 24, 2025
概括
微RNA-155 (miR-155) 通常通过降低血管激素II型1受体 (AT1R) 的调节来限制胎盘发育. 在小鼠中,miR-155的损失导致胎盘变大和胎儿生长受损,这表明miR-155的存在.
科学领域:
- 生殖生物学和发育科学.
- 分子内分泌学和微RNA研究.
- 遗传学和发育生物学.
背景情况:
- 微RNAs (miRNAs) 是胎盘发育的关键调节者.
- 众所周知,miR-155会影响胎盘发育,并向血管新生二型1型受体 (AT1R) mRNA.
- AT1R促进了繁殖和早期的胎盘生长.
研究的目的:
- 为了调查miR-155降低Agtr1mRNA表达,并损害胎盘发育的假设.
- 阐明miR-155在调节胎盘形态和功能的作用.
- 为了确定miR-155对热囊细胞行为的影响.
主要方法:
- 18.5.5.怀孕一天的野生型 (miR-155+/+) 和miR-155淘汰赛 (miR-155-/-) 小鼠的胎盘和胎儿的比较分析
- 评估胎盘形态,立体参数和miR-155和AGTR1 (mRNA和蛋白质) 的表达.
- 在体外研究中,使用使用miR-155模拟器治疗的HTR8/SVneo细胞来评估对热囊细胞增殖,迁移和入侵的影响.
主要成果:
- miR-155-/-母体表现出明显更重的幼,但胎盘体重和胎盘与胎盘体重比例没有变化.
- 来自miR-155-/-水的胎盘显示出更大的迷宫区域和改变的立体参数.
- 在miR-155-/-小鼠中观察到胎盘Agtr1mRNA和AGTR1蛋白水平增加,而体外miR-155模仿治疗减少了AGTR1mRNA和热囊细胞的增殖,迁移和入侵.
结论:
- miR-155可以减弱小鼠的胎盘发育,部分原因是它对AT1R通路的调节.
- 这些发现表明miR-155在平衡胎盘生长和功能方面起着至关重要的作用.
- miR-155充当了通过AT1R.Trophoblast扩散,迁移和入侵的负调节者.
相关概念视频
Hormonal Regulation
35.6K
The renin-aldosterone system is an endocrine system which guides the renal absorption of water and electrolytes, thus managing blood pressure and osmoregulation. Activation of the system begins in the kidneys with a small cluster of cells adjacent to the afferent and efferent blood vessels of the renal corpuscle. As the nephrons are filtering blood, juxtaglomerular cells monitor blood pressure. If they detect a decrease in pressure, they release the hormone renin into the bloodstream.
35.6K
Teratogenicity
3.9K
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
3.9K
Antihypertensive Drugs: Direct Renin Inhibitors
1.2K
The renin-angiotensin-aldosterone system (RAAS) is an intricate physiological pathway involving numerous enzymes and hormones, including renin, angiotensin-converting enzyme (ACE), angiotensin I and II, and aldosterone. Imbalances within this system increase the production of angiotensin II and aldosterone. Increased angiotensin II levels promote vasoconstriction and blood pressure elevation. Concurrently, higher aldosterone levels stimulate sodium and water reabsorption in the kidneys,...
1.2K
Regulation of Angiogenesis and Blood Supply
3.3K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K
Antihypertensive Drugs: Angiotensin II Receptor Blockers
2.4K
In the renin-angiotensin-aldosterone system, a hormone called angiotensin II plays a crucial role. It binds to the AT1 receptors in vascular smooth muscles coupled with Gq proteins. The activation of these receptors activates an enzyme called phospholipase C, which releases two molecules: inositol trisphosphate and diacylglycerol. These molecules cause a chain reaction that leads to the phosphorylation of myosin light chains and promotes interaction between actin and myosin, leading to smooth...
2.4K

