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毒素IR:一种精确的RNA-seq管道,用于高精度的毒素转录组概况,在odontobuthus doriae毒液腺体中得到验证

Mehran Ebadi¹, Maryam Naderi Soorki²

¹Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Scientific reports

|December 24, 2025

概括

毒素IR是一种新的RNA测序管道,可以准确分析复杂的毒素转录组. 这种工具增强了从有毒物种中发现新型治疗毒素的发现.

科学领域:

生物化学生物化学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

对像毒腺这样复杂组织的转录组分析是具有挑战性的,原因是组装错误,异构体多样性和注释偏差.
子毒含有具有生物医学潜力的生物活性,但它们的复杂性阻碍了准确的分析.

研究的目的:

开发和验证ToxIR,一个优化的RNA-seq管道,用于准确的毒素转录组分析.
改进低丰度毒素的检测,并高精度地分辨异型.

主要方法:

ToxIR 结合了深度测序,使用 rnaSPAdes 的 de novo 组装和定制的注释策略.
该管道包括质量控制 (FastQC,Trimmomatic),UniProt同质性搜索,以及结构分析 (SignalP,TMHMM,Pfam,InterProScan).该管道包括质量控制 (FastQC,Trimmomatic),UniProt同质性搜索,以及结构分析 (SignalP,TMHMM,Pfam,InterProScan).
它通过模块化设计,自动查询和SQLite集成来最大限度地减少错误组装和注释偏差.

主要成果:

毒素IR在Odontobuthus doriae毒液腺体中发现了378种假定毒素候选物.
这包括192个高度可靠的候选物和23种新型,分离的毒素.
确定的毒素包括180个通道,111个通道和69个通道毒素.

结论:

毒素IR为准确的毒素转录组分析提供了强大的框架,加速了毒素的发现.
管道提高了注释的精度,并使灵活的跨物种应用.
毒素IR 便于从复杂的毒源中识别潜在的治疗毒素.

关键词:

在新的组装 De novo.它们是Odontobuthus doriae.在RNA-seq管道上.子毒是一种子毒.这是ToxIRIR.毒素转录组学毒素转录组学

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