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Updated: Jan 7, 2026

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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NHEJ组件的冷-EM结构与核细胞组合在一起

Chloe Hall1, Philippe Frit2, Antonia Kefala-Stavridi3

  • 1Leicester Institute for Structural and Chemical Biology, Department of Molecular and Cell Biology, University of Leicester, Leicester, UK.

Nature communications
|December 24, 2025
PubMed
概括

研究人员可视化了关键蛋白质如何在核子体内识别DNA双链断裂 (DSB),揭示了DNA修复的机制. 这一发现对于了解癌症的发展和针对非同源端结合 (NHEJ) 的潜在疗法至关重要.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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