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在正常和病理条件下的猪肠微生物群的动态图

Inés García Viñado^1,2, Federico Correa², Paolo Trevisi²

¹Swine Research Unit, Agroscope, Route de La Tioleyre 4, 1725, Posieux, Switzerland.

Animal microbiome

|December 24, 2025

概括

这项研究使用可吞囊来追踪猪小肠微生物. 健康的猪从断奶到屠宰发生了变化,而ETEC感染对肠道微生物群的长期影响很小.

科学领域:

动物科学动物科学
微生物学微生物学
胃肠病学胃肠病学

背景情况:

小肠微生物群对宿主健康至关重要,但与便微生物群相比,它仍未得到充分研究.
测序和生物信息学的进步有助于分析与宿主相关的微生物群落.
可摄入的CapSa囊可以进行非侵入性的小肠微生物群采样.

研究的目的:

为了研究猪在整个生命周期中的小肠微生物组动态,使用安普利康测序.
评估肠毒性大肠杆菌 (ETEC) F4感染对小肠微生物群组成的长期影响.
为了评估CapSa囊对微生物群收集的疗效.

主要方法:

在多个时间点使用CapSa可摄入囊进行非侵入性微生物群采样.
采用AMPLICON测序来分析小肠微生物群体的组成.
对比健康猪中的微生物群概况与有ETEC F4感染史的猪.

主要成果:

坚果菌在小肠中占主导地位;乳酸菌和乳酸菌在断奶时丰富.
在健康的猪中,微生物的组成随着年龄和饮食的变化而变化;在屠宰时Streptococcaceae占主导地位.
ETEC感染导致乳酸菌种的丰富性降低,在屠宰时观察到的长期成分差异很小.

结论:

CapSa囊是监测猪小肠微生物群的有效工具.
猪小肠微生物群落从断奶后到屠宰时经历了动态变化.
ETEC感染引起了短暂的变化,对小肠微生物群产生了微妙的长期影响.

关键词:

囊采样采样囊采样采样在ETEC感染.微生物群中的微生物群小肠小肠小肠的小肠.

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