预激活时间通过STING抑制来确定流感的严重程度和病毒病原性
Tong Zhu1, Mengru Zhu1, Feiyu Lu2
1Department of Neonatology, Institute of Pediatrics, Children's Medical Center, First Hospital of Jilin University, Jilin University, Changchun, 130021, Jilin, China.
Scientific reports
|December 24, 2025
概括
细胞预刺激通过增加炎症细胞死亡,与抑制的STING激活相关,使流感的严重程度恶化. 这揭示了共感染病原发生的新型细胞内在机制.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 病毒学 病毒学
背景情况:
- 严重的流感通常涉及联合感染,但在免疫参与之前呼吸道上皮细胞内在反应尚不清楚.
- 研究了共感染中的免疫介导机制,但对流感病原发生的细胞预激活效应尚不清楚.
研究的目的:
- 为了研究呼吸道上皮细胞在预激活后的A型流感病毒 (IAV) 共感染期间的细胞内在反应.
- 阐明STING (干扰素基因刺激器) 途径和炎症细胞死亡在加剧流感严重性的作用.
主要方法:
- 在实验室模型中使用预激活的B16黑色素瘤细胞与CpG ODN,其次是IAV感染.
- 通过西部抹杀和RT-qPCR评估STING表达和激活.
- 在小鼠模型中进行了体内验证,并进行了鼻内CpG ODN预处理和IAV挑战,并补充了RNA-seq.
主要成果:
- 预激活在24小时内增强了IAV诱导的细胞死亡,独立于病毒复制或TLR9信号.
- 初始的STING激活随后抑制了STING酸化,并在IAV感染后增加了炎症细胞死亡.
- 在小鼠中进行CpG ODN预治疗导致死亡率增加和肺部损伤加重,RNA-seq表明皮रोप托斯参与.
结论:
- 细胞预刺激通过促进炎症性细胞死亡,加剧了流感的严重程度.
- 这种恶化在机制上与IAV随后抑制STING激活有关.
- 这项研究确定了一种新的细胞内在的致病机制,有助于共同感染的严重程度.
更多相关视频
04:47A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
Published on: February 23, 2018
8.1K
09:31Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
Published on: April 15, 2017
18.9K
相关概念视频
01:29Immune Response Against Viral Pathogens
1.6K
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
1.6K
02:28Leaky Scanning
5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K