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分子动力学研究双极脉冲下的等离子膜电穿孔

Fei Guo¹, Yue Zhuo¹, Xin Song¹

¹Institute of Ecological Safety, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.

Langmuir : the ACS journal of surfaces and colloids

|December 25, 2025

概括

双极脉冲 (BP) 应用序列影响细胞膜电穿孔 (EP). 超极化-去极化序列显示较高的穿孔率和较大的孔隙面积比去极化-超极化,影响EP结果.

科学领域:

生物物理学的生物物理.
计算生物学计算生物学
细胞膜动力学细胞膜动力学

背景情况:

双极脉冲 (BP) 对生物等离子体膜的作用的确切机制尚未完全理解.
血膜具有复杂的组成和不对称的脂质分布.

研究的目的:

系统地研究BP及其应用序列对等离子膜电穿孔 (EP) 的影响.
通过粗粒度分子动力学 (MD) 模拟,使用哺乳动物平均血模型分析BP效应.

主要方法:

使用马蒂尼框架进行粗粒度分子动力学 (MD) 模拟.
研究两种双极脉冲模式:去极化-超极化 (D-H BP) 和超极化-去极化 (H-D BP).
分析等离子膜电穿孔状态 (保持和重新结) 和穿孔区域.

主要成果:

观察到两个不同的电穿孔状态,保留和重新密封.
增加脉冲间隔长度减少了保持状态的频率,增加了恢复状态的频率.
超极化-去极化双极脉冲 (H-D BP) 导致与D-H BP相比,保持状态的频率更高,穿孔区域更大.

结论:

双极脉冲的应用序列是电穿孔过程中的一个重要因素.
在电孔过程中,BP序列可能会影响孔隙扩张和局部化.
需要进一步的研究,以充分阐明BP序列对EP的影响.

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