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孕产妇供应和胚胎激活:早期大西洋鱼 (Hippoglossus hippoglossus) 胚胎发生的转录基因动力学

Nils Niepagen¹, Francesca Bertolini², Leif Berg³

¹Department of Biology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.

Frontiers in cell and developmental biology

|December 25, 2025

概括

大西洋鱼胚胎的发育依赖于中芽细胞过渡 (MBT) 后的基因表达变化. 阶段解决的RNA测序揭示了轴形成和器官生成的关键路径,对于了解水产养殖中的卵和幼虫质量至关重要.

科学领域:

水产养殖是水产养殖的一种方式.
发展生物学发展生物学
基因组学就是基因组学.

背景情况:

蛋和幼虫的质量是大西洋白水产养殖的关键限制.
早期对受精卵的分子比较可能会错过发生在中芽细胞过渡 (MBT) 后的关键发育事件.

研究的目的:

为了研究大西洋鱼胚胎中分阶段解析的转录动态.
确定关键的发育途径和影响胚胎活力的基因表达变化.

主要方法:

大西洋鱼胚胎的阶段性RNA测序,从未受精的卵子到 бластопор关闭.
不同基因表达分析 (DESeq2) 和基因本体学 (GO) 丰富分析.
使用精选的轴模式基因组绘制转录动态图.

主要成果:

最小的转录变化发生在早期裂变阶段,由母体转录主导.
在MBT开始显著的转录转移,Wnt,BMP和Notch通路的上调,以及轴规范基因.
胃化涉及活跃的形态遗传过程,而后期阶段显示出血统差异化;轴模式涉及母体和胚胎因子.

结论:

轴形成和器官生成的决定性转录程序从MBT开始就会被激活.
早期阶段的比较不足以理解胚胎活力;包括MBT和胃化在内的阶段解决分析是必不可少的.
识别卵子和幼虫质量的转录基因标记需要全面的阶段解决分析.

关键词:

大西洋鱼大西洋鱼.蛋的质量蛋的质量胚胎发育过程中的胚胎.孕产妇的胚胎过渡孕产妇胚胎过渡转录组学转录组学是指转录组学.

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