多omics调查揭示了癌细胞进化在软细胞外基质上的分子决定因素
Sarthak Sahoo1, Sejal Khanna1, Ting-Ching Wang2
1Department of Bioengineering, Indian Institute of Science, Bengaluru, Karnataka, India - 560012.
bioRxiv : the preprint server for biology
|December 25, 2025
概括
癌细胞通过表观遗传变化沉默压力反应来适应软瘤微环境. 这促进了增殖和高适应性,揭示了癌症进化中的关键机制.
科学领域:
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 癌细胞适应物理瘤微环境驱动恶性瘤.
- 软细胞外基质 (ECM) 对瘤细胞施加选择压力.
- 软选择克隆的高适应性机制尚未完全理解.
研究的目的:
- 研究癌细胞适应软ECM的分子机制.
- 为了比较软选择的癌细胞种群与祖先种群.
- 了解在软选择克隆中驱动高健身的表观遗传修饰.
主要方法:
- RNA-sequencing (RNA-seq) 是一种RNA的测序方法.
- 使用测序 (ATAC-seq) 检测转化酶可访问的染色体.
- 减少表示双硫酸盐测序 (RRBS-seq)
- 基于in-silico机制的建模
主要成果:
- 软ECM上的祖先细胞表现出一种压力状态,细胞循环停止和代谢转变.
- 持续的文化选择了强大的繁殖表型,具有沉默的应激反应.
- 表观遗传修饰,包括DNA甲基化和减少染色质可访问性,是选择细胞的特征.
- 选择的细胞表现出高MYBL2和FAK水平,具有高YAP1核定位.
结论:
- 癌细胞通过协调的遗传和表观遗传机制在柔软的微环境中进化.
- 对祖先应激反应的表观遗传沉默对于高健身至关重要.
- 升高的MYBL2,FAK和YAP1是适应软ECM的癌细胞的关键特征.
关键词:
在ECM的硬度.表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.进化 进化 进化 进化 进化 进化 进化健身 适应 适应 适应 适应选择 选择 选择 选择软ECM是一种软的ECM.在 YAP/TAZ 信号系统中.更多相关视频
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