界面张力调节粘性微流体滴滴生成
Aarthi Namasivayam1,2, Christopher J Halbrook3,2,4, Elliot E Hui1,2
1Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.
bioRxiv : the preprint server for biology
|December 25, 2025
概括
使用微流体液滴生成用于药物查的3D组织模型是具有挑战性的,因为高粘度. 使用表面活性剂降低表面张力有效地克服了地下膜提取物中这种粘度问题.
科学领域:
- 生物技术是生物技术.
- 生物材料工程 生物材料工程
- 药物发现 药物发现 药物发现
背景情况:
- 哺乳动物细胞培养在纳米升液滴的细胞外基质是一个有希望的平台,用于高通量药物查.
- 与二维培养相比,3D组织模型提供了更具生理相关性的环境.
- 使用粘性材料 (如地下膜提取物) 产生微流体滴滴,带来了重大的技术挑战.
研究的目的:
- 通过使用高粘度底层膜提取物来研究微流体液滴生成的挑战.
- 确定优化滴滴生成的方法,用于3D组织模型应用.
- 探索表面张力和表面活性剂度在克服粘度限制中的作用.
主要方法:
- 使用T结微流体装置进行滴滴生成.
- 进行了参数研究,改变了表面活性剂度.
- 分析了与粘度和界面张力相关的滴状形成特征.
主要成果:
- 底层膜提取物 (例如Matrigel,Cultrex) 的高粘度显著复杂化了微流体滴滴的产生.
- 与高粘度相关的毛细血管数量的增加阻碍了稳定的滴滴形成.
- 通过添加表面活性剂来降低表面张力,有效地消除了高粘度的负面影响.
- 表面活性剂度是调节界面张力和实现优质滴滴生成的关键参数.
结论:
- 使用粘性底层膜提取物生成微流体滴滴可以通过控制界面张力来优化.
- 通过表面活性剂减少界面张力是一种可行的策略,可以在3D组织模型上实现高通量查.
- 这种方法促进了使用生物打印3D组织模型开发先进的药物查平台.
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