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Updated: Jan 7, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

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一个基本的核激素受体的约束性景观在C. 伊莱根斯 (elegans) 是一个词

Alexander L Sinks1, Masako Asahina2, Keith R Yamamoto2

  • 1Biology Department, Davidson College, Davidson, NC 28031.

bioRxiv : the preprint server for biology
|December 25, 2025
PubMed
概括

这项研究绘制了C. elegans中基本转录因子NHR-25的结合部位. 发现单个调节元件可以控制多个基因,揭示了复杂的基因调节.

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Transducer Mechanism: Nuclear Receptors

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
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