高纳引离离子选择性在激光吸附/电离质谱学中,使用固态调节的COF
Zhichao Yan1, Xiaoyan Wang1, Zhenxin Wang2
1Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.
Analytical chemistry
|December 25, 2025
概括
使用含硫的共价有机框架 (S-COFs) 的新方法增强了激光脱离电离质谱法 (LDI-MS),用于敏感的代谢物检测. 这种先进技术有助于高精度地诊断诸如型肝炎病毒 (HCV) 等疾病.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 生物化学 生物化学
背景情况:
- 激光消耗电离质谱法 (LDI-MS) 对于快速的代谢分析和诊断非常有价值.
- 开发新的基板对于提高LDI-MS灵敏度和选择性至关重要.
研究的目的:
- 开发一种使用硫含有共价有机框架 (S-COFs) 作为基质的先进LDI-MS方法.
- 增强对Na+而不是K+的选择性,以改善代谢物检测.
- 应用该方法用于临床诊断,特别是针对C型肝炎病毒 (HCV).
主要方法:
- 作为LDI基质的工程S-COF具有Na+的特定亲和力.
- 使用密度函数理论 (DFT) 计算来理解离子吸附.
- 应用S-COF辅助的LDI-MS用于血清代谢物分析.
- 使用机器学习算法 (SVM,NNW) 进行HCV诊断.
主要成果:
- S-COF基质显示有选择性的Na+结合,显著提高了像葡萄糖这样的代谢物的信号强度 ([M+Na]+与[M+K]+).
- 达到葡萄糖的检测极限为10 pg,远远超过传统矩阵.
- 成功区分了HCV患者和健康对照的血清代谢概况,准确度很高 (AUC=0.990).
- 确定了36种代谢物,其中5种在氨酸/甲氨酸途径中丰富,表明改变了氧化还原稳定性.
结论:
- 功能化COF的模块化设计为LDI基板开发提供了一个有前途的战略.
- 由S-COF辅助的LDI-MS为临床诊断和代谢研究提供了一个灵敏和多功能平台.
- 该研究突出了潜在的生物标志物和参与HCV病变的途径.
相关概念视频
01:15High-Resolution Mass Spectrometry (HRMS)
2.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.3K
01:08Matrix-Assisted Laser Desorption Ionization (MALDI)
910
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
910
01:19MALDI-TOF Mass Spectrometry
6.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.4K
01:13Mass Analyzers: Overview
1.5K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
1.5K
01:21Mass Spectrometry: Complex Analysis
1.5K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.5K
01:19Mass Analyzers: Common Types
1.3K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
1.3K