甲胺通过重塑微生物群落并促进横向基因转移来驱动活性污泥中的抗生素耐药性
Jiaming Yi1, Zhengtao Li1, Xuezhu Han1
1Zhejiang Key Laboratory of Solid Waste Pollution Control and Resource Utilization, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, China.
Journal of hazardous materials
|December 25, 2025
概括
废水中的甲胺 (MET) 污染会在有氧颗粒污泥中放大人类细菌病原体和抗生素耐药性基因 (ARG). 这种新出现的污染物促进了病原体的生长和水平基因转移,增加了抗生素耐药性的风险.
科学领域:
- 环境微生物学环境微生物学
- 污水处理 污水处理 污水处理
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 有氧颗粒污泥 (AGS) 含有人类细菌病原体 (HBPs) 和抗生素耐药性基因 (ARGs).
- 新出现的污染物甲胺 (MET) 恶化了抗生素耐药性,并破坏了废水处理过程.
- 对MET对微生物群落和活性污泥中ARG传播的影响机制尚不清楚.
研究的目的:
- 研究MET对抗生素压力下的AGS中微生物群落和ARG的影响.
- 阐明能量代谢和社区竞争在MET影响中的作用.
- 了解MET对ARG传播和水平基因转移的贡献.
主要方法:
- 暴露于MET和抗生素的AGS的元基因组分析.
- 同时发生的网络分析,以确定HBPs,ARGs,毒性因子和移动遗传元素之间的相关性.
- 自然转化和结合实验,以评估MET在水平基因转移中的作用.
主要成果:
- 暴露于MET显著丰富HBPs和多药耐药ARGs.
- 27个高风险HBP与ARG,毒性因子和移动遗传元素有很强的相关性.
- 在特定的HBPs中,MET增强了ATP的产生,促进了ARG的积累和水平基因转移.
结论:
- 通过丰富病原体和促进ARG转移,MET加剧了AGS中抗生素耐药性的风险.
- 在废水处理生态系统中,MET是ARG传播的重要驱动因素.
- 了解MET的影响对于管理废水处理中的阻力至关重要.
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