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PRDM14通过在初始阶段增强氧化酸化来促进牛体干细胞重编程

Qingqing Wei¹, Wenhui Li¹, Guina Cui¹

¹Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science, Shandong Agricultural University, Taian, China.

|December 25, 2025

概括

通过在早期阶段促进氧化酸化,PRDM14可以提高牛体细胞重编程的效率. 这种转录因子对于多能性重新获得和保持牛诱导的多能干细胞身份至关重要.

科学领域:

干细胞生物学干细胞生物学
生殖生物学生殖生物学
分子生物学分子生物学

背景情况:

牛诱导的多能干细胞 (biPSCs) 对再生医学和动物育种具有前景.
低的重新编程效率和不太了解的机制阻碍了biPSC应用程序.
转录因子PRDM14在牛体细胞重编程中的作用在很大程度上尚未被探索.

研究的目的:

研究PRDM14在牛体细胞重编程中的作用.
阐明PRDM14影响多能性的机制.
评估PRDM14在增强双PSC生成和维护方面的潜力.

主要方法:

在牛胚胎纤维细胞 (BEFs) 中,PRDM14的过度表达和抑制.
使用有或没有PRDM的OSKM因素生成双PSC14.
型定型,多能性标记分析,分化试验,转录组分析,RT-qPCR和ATP含量检测.

主要成果:

通过OSKM因素,PRDM14显著提高了BEF重编程效率.
在早期重编程阶段,PRDM14促进氧化酸化 (OXPHOS).
在双PSC中,PRDM14可以调节NANOG,并降低LIN28,DNMT1/3B和TET1/2/3的调节.

结论:

PRDM14是一种关键的转录因子,对于牛体细胞中有效的多能性再获取至关重要.
PRDM14通过调节代谢途径 (OXPHOS) 和表观遗传调节器来增强双PSC生成.
PRDM14在保持牛诱导多能干细胞的身份和多能性方面发挥着至关重要的作用.

关键词:

诱导的多能干细胞干细胞氧化酸化是一种氧化酸化.在 PRDM14 中,身体细胞正在重新编程.

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