生物标志物 生物标志物
Hanna Huber1, Wagner Scheeren Brum2, Laia Montoliu-Gaya2
1University of Gothenburg, Gothenburg, Sweden.
Alzheimer's & dementia : the journal of the Alzheimer's Association
|December 25, 2025
概括
从毛细血管血液中干燥的血斑 (DPS) 提供了一种简单的,独立于温度的方法来检测阿尔茨海默病 (AD) 生物标志物. 这种方法提高了人口查和研究的可访问性,改善了AD诊断和研究包容性.
科学领域:
- 神经科学是一个神经科学.
- 生物化学 生物化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 针对阿尔茨海默病 (AD) 的基于血液的生物标志物是越来越有价值的临床工具.
- 提高像pTau217这样的生物标志物的可访问性可以提高人口查和研究包容性.
- DROP-AD项目开发了一种替代性血液采集方法,以克服传统的挑战.
研究的目的:
- 从毛细血管血液中检测阿尔茨海默病 (AD) 生物标志物的可行性和准确性,使用干燥血斑点 (DPS).
- 评估毛细血管DPS生物标志物与传统静脉血生物标志物之间的相关性.
- 为了确定毛细血管DPS pTau217在识别AD病理学的诊断性能.
主要方法:
- 在八个中心招募了344名参与者,收集配对的静脉EDTA血和毛细血管指纹棒衍生的DPS.
- 在没有温度控制的情况下运送了毛细血管样本,使用自定义协议提取它们,并使用ALZpath pTau217 Simoa测试进行分析.
- 利用CSF生物标志物进行Aβ阳性分类,并进行认知评估 (MMSE,CDR-Global),分析数据与相关性,线性模型和ROC曲线分析.
主要成果:
- 毛细管DPS pTau217与静脉血pTau217 (rs=0.74) 强烈相关,并与认知得分和年龄的反射关联.
- 毛细管DPS pTau217显示了AD病理学的0.863的分辨准确度,受影响个体的升高为198%.
- 在NfL (rs=0.83) 和GFAP (rs=0.773) 中,毛细血管DPS和静脉血之间观察到强烈的相关性.
结论:
- 使用DPS从毛细血管血液中检测和量化AD生物标志物是可行的和准确的.
- 这种简单的,温度独立的方法可以帮助识别患AD高风险的个体.
- 该方法扩大了对生物标志物测试的访问,特别是在研究环境中.
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