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Updated: Jan 7, 2026

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Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
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在MIL-101上的联体介导蛋白冠状病毒 (Ligand-Mediated Protein Corona) 通过结构-蛋白-细胞级联控制细胞毒性
Yi-Bo Yuan1, Miao-Miao Yin1, Zhi-Yu Zuo1
1Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi 435002, PR China.
Biomacromolecules
|December 25, 2025
概括
金属有机框架 (MOF) 的连接体功能控制蛋白质冠状形成. 这影响MOF生物相容性和癌细胞毒性,为生物医学应用提供设计策略.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 金属有机框架 (MOF) 的生物医学应用取决于它们的表面连接体和体内形成的蛋白质冠状.
- 了解连接体如何影响蛋白质冠状形成和随后的生物相互作用至关重要,但人们对此了解甚少.
研究的目的:
- 调查MIL-101 (Fe) 的连接体功能化如何影响冠状蛋白组成和随后的细胞反应.
- 阐明不同蛋白质冠状体调节MOF生物相容性和细胞毒性的机制.
主要方法:
- 使用电子捐赠和提取组合成三个功能化的MIL-101 (((Fe)) 衍生物 (H-, NH2-, NO2-MIL-101 (((Fe)) .
- 使用表面连接体电子效应,评估MOF结合亲缘关系和与血清蛋白 (HSA,转激素) 的相互作用机制.
- 使用正常肝细胞和4T1癌细胞进行体外细胞研究,以评估生物相容性和细胞毒性.
主要成果:
- 体电子性质 临界调节的蛋白质结合亲和力 (H >= NH2 > NO2) 和相互作用机制 (结合与静电).
- 在正常肝细胞中,HSA冠状体增强了MOF生物相容性.
- 转移素 (TRF) 冠状腺促进MOF吸收,产生活性氧物种,以及癌细胞中的线粒体损伤,增加细胞毒性.
结论:
- 连接体功能化是编排蛋白质冠状结构和随后的生物级联的关键策略.
- 这项研究为设计具有定制生物医学性能的MOF提供了机制的理解.
- 这些发现为生物医学应用中精确控制MOF相互作用提供了设计策略.
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