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circDCBLD2通过miR-26a-5p/PTEN轴调节Th1/Th2免疫平衡

Yue Zhao¹, Guangling Liu¹, Rui Li²

¹Department of Pediatrics, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China.

Acta biochimica et biophysica Sinica

|December 26, 2025

概括

循环RNA DCBLD2 (circDCBLD2) 通过促进Th1细胞和减少Th2细胞,有助于恢复喘中的免疫平衡. 这一发现为儿童喘治疗提供了一个新的治疗点.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
呼吸系统医学呼吸系统医学

背景情况:

喘是儿童常见的慢性呼吸道疾病.
恢复Th1/Th2免疫平衡是喘免疫疗法的关键重点.
循环RNAs在调节喘免疫平衡中的作用需要进一步研究.

研究的目的:

研究circDCBLD2在维持喘中的Th1/Th2免疫平衡中的功能和机制.
探索circDCBLD2作为喘潜在的治疗点.

主要方法:

分析了来自喘患者和小鼠模型的CD4+T细胞和PBMC中的circDCBLD2表达.
使用流细胞计和ELISA来评估circDCBLD2过度表达对Th1/Th2细胞比例和细胞因子水平的影响.
通过在OVA诱导的喘小鼠模型中进行救援实验,研究涉及circDCBLD2,miR-26a-5p和PTEN的分子机制.

主要成果:

在喘患者和小鼠的CD4+T细胞和PBMC中,CircDCBLD2的下调.
CircDCBLD2的过度表达促进了Th1细胞分化和Th1细胞因子的产生,同时抑制了Th2细胞和细胞因子.
通过调节miR-26a-5p/PTEN轴,CircDCBLD2减轻了小鼠的喘症状,纠正了Th1/Th2不平衡.

结论:

CircDCBLD2在维持Th1/Th2免疫平衡方面发挥着至关重要的作用.
CircDCBLD2通过miR-26a-5p/PTEN通路作用,调节喘中的T细胞反应.
CircDCBLD2代表了喘治疗的一个有前途的治疗标.

关键词:

Th1/Th2 的不平衡.喘喘是一种圆圈DCBLD2 在线观看在 miR-26a-5p/PTEN 轴上

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