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BPSS:用于细菌序列选择的下一流管道,以检测蛋白质多样性

Sylvère Bastien^1,2, Pauline François^1,2, Sara Moussadeq^1,2

¹CIRI, Center for Integrative Research in Infectious diseases and Immunology, Université de Lyon, Inserm U1111, Université Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, Lyon, France.

Bioinformatics (Oxford, England)

|December 26, 2025

概括

细菌序列选择 (BPSS) 是一个新的管道,可以创建最小的序列列表,以检测所有蛋白质变体. 该工具解决了分析高细菌序列变异性的有效方法的缺乏问题.

科学领域:

微生物学微生物学
生物信息学是一种生物信息学.
计算生物学计算生物学

背景情况:

由于快速突变积累和选择压力,细菌序列的变异性很高.
创建非冗余蛋白质变体数据库的现有工具通常是低效的或不可用.
了解等位基变异性对于细菌研究和诊断至关重要.

研究的目的:

开发一种自动化和计算效率高的工具,用于构建非冗余蛋白质变体数据库.
为了应对细菌群体高序列变异性的挑战.
为了方便检测目标蛋白质的所有变体.

主要方法:

一个名为细菌序选择 (BPSS) 的Nextflow管道的开发.
BPSS定义了一组最小的序列,以表示蛋白质基变异性.
该管道以开源代码和Docker容器的形式提供.

主要成果:

BPSS成功生成了一个最小的序列列表,用于全面检测变种.
该管道为管理细菌蛋白质多样性提供了有效的解决方案.
现在可以自动构建覆盖等位基变异性的非冗余数据库.

结论:

BPSS提供了一种高效的计算解决方案,用于分析细菌蛋白质序列的可变性.
该工具有助于全面检测蛋白质变体,这对研究至关重要.
开放的BPSS可用性促进了细菌基因组学更广泛的采用和进步.

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