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相关概念视频

DNA Isolation01:24

DNA Isolation

44.4K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
44.4K
DNA Isolation01:34

DNA Isolation

198.9K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
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相关实验视频

Updated: Jan 7, 2026

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
06:53

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

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使用Filterprep的等离子体DNA净化与可选的内毒素清除步骤.

Yu-Qian Lin1, Yung-Chun Shih1, Chung-Te Chang1

  • 1Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan.

Bio-protocol
|December 26, 2025
PubMed
概括

这项研究引入了一种经过修改的Filterprep方法,用于快速,低成本的等离子体DNA净化. 增强的协议提供了高产量和纯度,可选的步骤是降低敏感应用的内毒素水平.

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 等离子体DNA净化对于分子生物学应用至关重要.
  • 现有的方法可能是昂贵的,耗时的,或依赖于专有试剂.
  • 内毒素污染可以干扰敏感的下游应用.

研究的目的:

  • 提出一种改进的,透明的,具有成本效益的Filterprep协议用于等离子体DNA净化.
  • 加入一个可选的内毒素降低步骤.
  • 提供一种高产量,高纯度的DNA净化方法,适应各种实验室环境.

主要方法:

  • 修改了Filterprep协议,结合了乙醇沉和单列过.
  • 消除杂热盐和结合的作用.
  • 可选的Triton X-114洗步骤用于减少内毒素.

主要成果:

  • 实现了高纯度的等离子体DNA,产量高达商业套件的五倍.
  • 在常规条件下,协议在约40分钟内完成.
  • 已证明适用于分子克隆,PCR,测序和其他应用.

结论:

  • 经过修改的Filterprep方法是一种简单,快速和具有成本效益的替代品,用于等离子体DNA净化.
关键词:
减少内毒素的减少乙醇沉 乙醇沉是一种这是一个模块化协议.分子克隆是一种分子克隆.质粒DNA净化净化方法旋转列的旋转列是一个旋转列.转染级DNA是转染级的DNA.

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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
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相关实验视频

Last Updated: Jan 7, 2026

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

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Air-sampled Filter Analysis for Endotoxins and DNA Content

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  • 该协议是模块化的,可扩展的,可适应各种规模和下游应用.
  • 可选的内毒素降低步骤增强了它对敏感实验的实用性.