优化蓝色便:一种经过验证,具有成本效益的方法来测量全肠传输时间.
Cyra Schmandt1,2, Julia Trunz1,3, Claudio Perret1,3
1Swiss Paraplegic Research, Nottwil, 6207, Switzerland.
MethodsX
|December 26, 2025
概括
这项研究优化了蓝色染料方法来测量全肠传输时间 (WGTT),提供了一个负担得起和最少侵入性的替代方案. 使用囊与染料,米饼和液体的标准化协议确保了准确的WGTT评估.
科学领域:
- 胃肠病学 胃肠病学
- 药理动力学 药理动力学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 全肠传输时间 (WGTT) 对于评估胃肠道健康至关重要.
- 传统的WGTT方法通常是昂贵或侵入性的,限制了它们的广泛使用.
- 存在需要负担得起的,最少侵入性的WGTT测量技术.
研究的目的:
- 优化和验证用于WGTT测量的蓝色染料方法.
- 为WGTT评估建立一个标准化,实用和准确的协议.
- 评估不同的输送方法和染料度,以获得最佳的WGTT测定.
主要方法:
- 这项研究使用了"霍林格Farbpulver Blau" (食品颜色E131,E132) 在30毫克至241毫克的度.
- 测试了四种输送方法:囊与液体,熊,蛋糕,囊与米饼干和液体.
- 在各种传递方法和染料度中测量了WGTT,以确定限制和最佳参数.
主要成果:
- 不同的输送方法和染料度产生了从18到29小时的WGTT.
- 含有液体的囊显示出不一致的运输时间,熊导致染色,蛋糕很容易腐烂.
- 最佳的方案是使用一个含有60毫克染料的囊,与饼和液体一起服用,确保准确性和方便性.
结论:
- 标准化的蓝色染料方法为WGTT测量提供了一种有效,负担得起和最少侵入性的方法.
- 这种优化的方法适用于大规模的人口研究和临床应用.
- 已建立的协议提供可靠和可重复的WGTT测量.
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