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通过使用代谢学和转录学分析,对宁克什米尔山羊的克什米尔生产绩效差异的综合分析

Bing Luo¹, Meiqi Wang¹, Di Han²

¹College of Animal Science and Veterinary Medicine, Jinzhou Medical University, Jinzhou, China.

Frontiers in veterinary science

|December 26, 2025

概括

具有多年长毛 (PT-LCG) 特性的宁克什米尔山羊通过增强脂质代谢和重塑细胞外基质来维持持续的克什米尔生长. 这项研究揭示了改善喀什米尔产量的关键分子机制.

科学领域:

动物遗传学和动物基因组学
皮肤病学和皮肤生物学
代谢学和转录学.

背景情况:

宁克什米尔山羊是中国有价值的双重用途品种.
选择性育种改善了喀什米尔产量,但仍有进一步增强的潜力.
存在不同的多年性 (PT-LCG) 和季节性 (ST-LCG) 长毛特征,其潜在的分子机制尚不清楚.

研究的目的:

阐明宁喀什米尔山羊多年性 (PT-LCG) 和季节性 (ST-LCG) 长毛特征的分化分子机制.
确定关键的基因和代谢物,涉及到长时间的毛囊活动和喀什米尔生产.
为分子育种策略提供见解,以提高喀什米尔产量和质量.

主要方法:

皮肤组织的比较分析PT-LCG和ST-LCG山羊在catagen阶段.
二次毛囊 (SHF) 的组织学检查.
非向的代谢分析 (GC-MS) 和转录组测序 (RNA-seq).
综合多主题分析和定量实时PCR (qRT-PCR) 验证.

主要成果:

在SHF中没有显著的结构差异,但在PT-LCG中SHF活性较高.
代谢学发现了92种差异表达代谢物 (DEM),富含脂质,氨基酸和能量代谢途径.
转录学揭示了145个差异表达基因 (DEGs),富含脂质代谢,细胞外矩阵 (ECM) 组织和炎症途径 (例如,PPAR,IL-17).
综合分析确定了一种代谢物-基因网络,突出显示了脂质代谢,能量供应和ECM重塑的作用.

结论:

通过增强的脂质合成/储存和ECM重塑,PT-LCG山羊可以维持持续的喀什米尔生长.
调节炎症信号,例如IL-17通路,对于维持PT-LCG中的卵泡平衡至关重要.
这些发现为分子育种提供了有价值的目标,以改善山羊的喀什米尔生产.

关键词:

宁的喀什米尔山羊是一头山羊.喀什米尔生产表现的表现脂质新陈代谢脂质新陈代谢代谢生物组的代谢生物组转录组学转录组学是指转录组学.

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