功能性表征mpdh作为一个关键脱水酶在M.紫色YY-1中的氨酸生物合成
Sisi Feng1, Maolin Liu1, Xiaohui Wang1
1College of Food Engineering, Yantai Key Laboratory of Nanoscience and Technology for Prepared Food, Yantai Engineering Research Center of Food Green Processing and Quality Control, Ludong University, Yantai, Shandong 264025, PR China.
Food chemistry
|December 26, 2025
概括
破坏Monascus purpureus YY-1中的mpdh基因显著降低了有毒氨酸的产量和Monascus azaphilone颜料 (MonAzPs) 的产量. 这也通过重定向代谢资源来增强真菌生物量.
科学领域:
- 微生物学 微生物学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 莫纳斯克物种是传统的发酵生物,产生有价值的颜料,但也产生有毒的氨酸.
- 氨酸是一种毒性菌毒素,具有重大健康和经济影响.
- 了解生物合成途径对于控制菌毒素生产至关重要.
研究的目的:
- 为了研究Monascus purpureus YY-1中mpdh基因的功能.
- 阐明mpdh在氨酸和Monascus azaphilone颜料 (MonAzPs) 生物合成中的作用.
- 探索mpdh破坏对真菌新陈代谢和生长的影响.
主要方法:
- 基因淘汰创建一个 Δmpdh 菌株的莫纳斯科紫色YY-1.
- 发酵和生物质,氨酸和MonAzPs的分析.
- 转录组分析以确定差异表达基因 (DEGs).
- 代谢途径分析侧重于乙-CoA.
主要成果:
- 与野生类型相比,Δmpdh菌株显示生物质增加 (增强生长).
- 在Δmpdh菌株中,氨酸的产量减少了79.3%,MonAzPs的产量减少了31.4%.
- 转录组数据表明,氨酸生物合成集群中的基因下调,包括多基酸合成酶基因.
- 破坏mpdh导致乙-CoA水平降低,损害了二次代谢物生产,有利于能量代谢.
结论:
- mpdh基因通过催化关键脱水阶段,在氨酸生物合成途径中发挥关键作用.
- mpdh 干扰将乙-CoA 从二次代谢重定向到初级代谢,增强生物质.
- 针对mpdh提供了一种潜在的策略来控制蒙纳斯发酵中的氨酸生产.
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