在Stichopus monotuberculatus的肠道再生过程中,肠道微生物组的继承和基于培养的功能查
Xinyuan Yang1, Rong Cao2, Chenghao Jia2
1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China; School of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Marine environmental research
|December 26, 2025
概括
海肠道微生物组在压力诱导的内脏剥离后恢复. 研究人员确定了关键的细菌和消化酶,为益生菌提供了帮助可持续水产养殖和海洋生态系统健康的潜力.
科学领域:
- 海洋生物学 海洋生物学
- 微生物学 微生物学
- 水产养殖科学 水产养殖科学
背景情况:
- 压力诱导的内脏剥离会对海黄的生存和生长产生负面影响.
- 肠道微生物组在宿主健康和再生中起着至关重要的作用.
- 微生物组调制是一种有前途的策略,可以改善内脏切割后的恢复.
研究的目的:
- 为了研究在Stichopus monotuberculatus的肠道再生过程中肠道微生物社区的继承.
- 为了将具有消化酶活动的本土肠道细菌分离出来,用于潜在的益生菌应用.
- 了解海黄瓜再生过程中的宿主-微生物-环境相互作用.
主要方法:
- 16S rRNA基因测序以分析微生物社区动态.
- 文化依赖的分离技术,以获得本地肠道细菌.
- 细菌分离物的酶性选,以检测氨基酶,脂酶和细胞酶活动.
主要成果:
- 在不同再生阶段观察到不同的肠道微生物群落.
- 主要属Delftia和Ruegeria,在珊瑚礁中普遍存在,在内脏切割后反弹.
- 获得了163个细菌分离物,包括潜在的新物种,其中几个表现出多酶活性.
- 普里斯蒂亚巨, Bacillus stratosphericus 和 Lysinibacillus fusiformis 显示出显著的消化酶生产.
结论:
- 海肠道微生物组在再生过程中经历了显著的变化,受宿主选择和殖民的影响.
- 本土细菌具有功能性消化酶活动,突出显示它们作为益生菌的潜力.
- 这项研究提供了关于海肠道微生物组动态和可持续水产养殖资源的见解.
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