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Pseudomonas putida JM37作为一种用于乙烯基醇上循环的新型细菌底盘

Francisco J Molpeceres-García¹, Alejandro García-Miró¹, Elvira Mateos-García¹

¹Department of Biotechnology, Center for Biological Research Margarita Salas, Spanish National Research Council (CIB-CSIC), 28040 Madrid, Spain.

Bioresource technology

|December 26, 2025

概括

Pseudomonas putida JM37有效地将乙烯基醇 (EG) 代谢为生物生产. 这种新型的底盘使得多基酸盐的积累和有价值的酶的表达成为可能,从而促进了微生物的上循环.

科学领域:

微生物学微生物学
合成生物学合成生物学
生物技术是生物技术.

背景情况:

乙烯糖醇 (EG),一个关键的PET单体,是微生物上循环的目标.
有限的生物有效地代谢EG,需要新的微生物底盘.

研究的目的:

为了研究Pseudomonas putida JM37作为EG价值化的新型细菌底盘.
探索JM37的代谢和生物技术潜力,以实现可持续的生物生产.

主要方法:

在EG上种植JM37作为唯一的碳来源.
基因组测序和定向突变发生,以了解EG代谢.
分析聚基酸盐 (PHAs) 的积累和异质基因表达.

主要成果:

JM37在EG上表现出强的增长,优于其他Pseudomonas菌株.
基因分析显示,氧酸盐同化途径的冗余性有助于EG代谢.
积累的中链PHA (mcl-PHAs),富含EG的C10单体.
成功表达和分泌一个PET-水解酶和一个violacein生物合成操作子.

结论:

P. putida JM37 是一种用于可持续乙烯糖醇上循环的多功能底盘.
JM37提供了一个有前途的平台,通过微生物价值化循环生物生产.

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