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相关概念视频

Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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RNA Structure01:23

RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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RNA Structure01:19

RNA Structure

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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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The Nucleolus02:55

The Nucleolus

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The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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Transcription01:10

Transcription

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Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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Transcription01:17

Transcription

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Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
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设计者RNA纳米结构在人类细胞核内共同转录和自我组装.

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概括

研究人员开发了自组装RNA纳米结构,用于在人类细胞中输送核. 这些遗传编码的纳米网为先进的生物应用提供可编程的几何和本地化.

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科学领域:

  • 合成生物学 合成生物学
  • 分子生物学分子生物学
  • 纳米技术纳米技术

背景情况:

  • 使用核酸纳米结构与细胞过程的接口和调节具有挑战性.
  • 核输送和合成纳米结构在真核细胞中的保留是重要的障碍.

研究的目的:

  • 为基因编码,自组装RNA纳米结构提供一个平台.
  • 为了证明它们的共同转录性生产,核组装和活人细胞内的功能整合.

主要方法:

  • 单链RNA的共同转录折叠成定义的纳米结构 (环,带,纳米网).
  • 使用原子力显微镜进行体外验证.
  • 光吸光体和RNA传感能力的功能集成.
  • 在活体中在活人细胞中进行体内示范,使用共聚焦活细胞成像和传输电子显微镜.

主要成果:

  • 在体外验证的可编程几何结构 (环,带,纳米网) 的RNA纳米结构的形成.
  • 在活人细胞核内成功的共同转录生成和RNA纳米网的组装.
  • 在核中证明了明确的纳米结构模式的保留.
  • 在纳米结构中,aptamer和传感能力的功能集成.

结论:

  • 建立了一个基因编码的,自我组装的RNA纳米结构系统.
  • 证明了可编程几何和核定位能力.
  • 提供了基于RNA的纳米设备的基础,用于研究活细胞和组织中的生物特性.