一个高效的转录末端映射工具Hiten揭示了ZmRNase II和ZmPPR67在玉米器官RNA处理和稳定性中的功能
Zi-Wei Qian1,2, Hui Sun1, Feng-Rui Chang1
1Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
The New phytologist
|December 26, 2025
概括
我们开发了Hiten,这是一个有效的工具,用于绘制植物器官中的RNA末端. 希坦发现了ZmRNase II的存在.
科学领域:
- 植物分子生物学 植物分子生物学
- 有机细胞基因表达
- 在RNA生物学,RNA生物学.
背景情况:
- 植物有机细胞RNA末端成熟和稳定机制尚不清楚.
- 对于有机细胞转录,缺乏有效的RNA末端映射方法.
- 准确的测绘对于理解质体和线粒体中的基因表达调节至关重要.
研究的目的:
- 开发一种高通量工具,用于绘制有机细胞RNA5'和3'末端的映射.
- 在玉米中对有机细胞多氨酸尾巴的特征.
- 在器官RNA处理中识别RNA基质和ZmRNase II和ZmPPR67的功能.
主要方法:
- 开发Hiten (高通量转录端映射) 工具.
- 在体外RNA循环化,下一代测序和circRNA识别算法 (MeCi) 的整合.
- 应用Hiten来绘制细胞器RNA末端的地图,并分析玉米中特定酶的RNA基质.
主要成果:
- 在玉米中,Hiten系统地绘制了有机细胞mRNA和ncRNA的5'和3'末端.
- 转录启动是质体和线粒体RNA的5'-end形成的主要因素.
- ZmRNase II突变导致叶绿体和线粒体RNA中的3'-扩展;ZmPPR67突变导致5'末端截断的atp9mRNA的积累.
结论:
- 希是绘制器官RNA末端和选RNA基质的有效工具.
- ZmRNase II主要在质细胞RNA3'-end成熟过程中起作用.
- ZmPPR67通过保护其5'末端来稳定线粒体atp9mRNA.
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