Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Triphase Interfacial Nucleation Enables Microneedle-Templated Metal-Organic Framework Coating for <i>In Situ</i> DNA Capture and Release.

Analytical chemistry·2026

Same author

A Near-Infrared-Triggered Luminescence-Activated System for In Vivo Biomacromolecular Tagging and Photocatalytic Crosslinking for Large-Scale Investigation of RNA-Protein Complexes in Living Mice.

Journal of the American Chemical Society·2026

Same author

Octadecylamine-Modified CuO NPs Enabling Highly Selective <i>In Vivo</i> Ascorbic Acid Potentiometric Detection with Enhanced Sulfide Tolerance.

ACS sensors·2026

Same author

Effect of insoles with different cushioning properties on knee impact after radial tear of posterior horn of medial meniscus: a finite element analysis.

BMC musculoskeletal disorders·2026

Same author

Simulated microgravity-driven mechanical unloading rescues PIEZO1-overexpression-induced growth plate ossification and retards adolescent idiopathic scoliosis.

NPJ microgravity·2026

Same author

Distribution network risk prediction based on data mining and improved PSO fused with SVM.

Scientific reports·2026

Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026

Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026

Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026

Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026

Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026

Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026

查看所有相关文章

Search research articles

相关实验视频

Updated: Jan 7, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

以层次工程设计的菌体激活点击接口,用于超高效的细胞捕获.

Huida Li¹, Rui Wang¹, Fengting Jia¹

¹Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

Analytical chemistry

|December 27, 2025

概括

此摘要是机器生成的。

这项研究引入了一种新的多尺度细胞捕获接口 (PACE-Chip),可以显著提高细胞结合强度和动力学. 工程芯片有效地从复杂的样本中分离目标细胞,优于传统的2D方法.

更多相关视频

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Published on: October 19, 2018

相关实验视频

Last Updated: Jan 7, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Published on: August 19, 2012

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture CARIC Strategy

Published on: October 19, 2018

科学领域:

生物材料工程生物材料工程
细胞生物学细胞生物学
微流体学微流体学

背景情况:

在流动下有效的细胞粘附对于生物过程和人工细胞捕获系统至关重要.
现有的方法主要集中在2D中的分子水平增强,忽视了多尺度策略.
同时增强细胞表面碰撞频率和跨空间尺度的结合概率尚未得到充分探索.

研究的目的:

开发一个多尺度,层次工程细胞捕获接口,以协同增强细胞结合.
研究整合分子,微观和宏观特征对细胞捕获效率的影响.
为了优化菌体支架的长度,以提高细胞捕获性能.

主要方法:

设计了一个多层次的层次接口,PACE-Chip,集成点击化学,M13菌体支架和鱼骨结构.
评估了PACE芯片与2D对应器的结合强度和动力学.
评估了从复杂的血液基质中分离细胞的效率.
分析了菌体长度对结合性能的影响.

主要成果:

该PACE芯片显示结合强度增加了294%,结合动力学加速了181倍.
从复杂的血液基质中实现了高效的目标细胞隔离,直到单细胞水平.
鉴定出过多的菌体长度会由于热和结构性惩罚而对性能产生负面影响.

结论:

多层次的层次工程方法协同增强了细胞结合动力学和亲和力.
与传统的2D接口相比,PACE-Chip提供了一个高效的细胞隔离的优越平台.
在多价值和结构稳定性之间存在一个多尺度的权衡,需要优化菌体支架的长度.