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在Bacillus subtilis中为蛋白质胺酶生产提供蛋白质介导的蛋白酶激活

Xin Geng^1,2, Maofang Teng^1,2, Qinghua Li^1,2

¹Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.

Journal of agricultural and food chemistry

|December 27, 2025

概括

一种新型的中介激活 (IMA) 系统使得食品添加剂蛋白质胺酶 (PG) 的蛋白质酶独立生产成为可能. 这种方法在Bacillus subtilis中实现了高PG活性,为前酶激活提供了新的策略.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
食品科学食品科学食品科学

背景情况:

蛋白质氨酸酶 (PG) 是一种具有除化特性的新型食品添加剂.
PG自然表达为一种需要蛋白酶激活的前酶.
蛋白酶依赖激活对高效的PG生产构成挑战.

研究的目的:

开发一种因特因介导激活 (IMA) 系统,用于蛋白酶独立的PG生产.
为了评估PG激活不同整数的效率.
为了优化Bacillus subtilis的PG生产.

主要方法:

在大肠杆菌中构建的融合前酶与整蛋白.
在体外评估C端激活效率.
精选的Mth RIR1和Mxe GyrA蛋白质用于Bacillus subtilis系统的构建.
优化了促进剂,并使用了Bacillus subtilis WB600.00中的料批发发酵.

主要成果:

在大肠杆菌中成功表达了融合前酶.
确定了Mth RIR1和Mxe GyrA作为有效的对象.
在Bacillus subtilis WB600.00中达到37.2U/mL的PG活性.
已证明的一步,不依赖蛋白酶的PG生产.

结论:

IMA系统提供了一种有效的生产蛋白质胺酶的方法.
这种方法消除了PG激活中蛋白酶的需要.
开发的策略为激活其他益酶提供了一个替代方案.

关键词:

这种细菌是 Bacillus subtilis.没有任何的意义,没有意义.蛋白酶激活的方法蛋白质-谷氨酸胺酶的使用

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