无时无刻的BmaaNAT轴调节丝虫,Bombyx mori的温度依赖的隔膜可塑性
Lulu Liu1, Chunyan Yang1, Lu Zheng1
1State Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, The Ministry of Agriculture and Rural Affairs, Yibin Academy of Southwest University, Southwest University, Chongqing, China.
Insect science
|December 27, 2025
概括
循环时钟基因调节了昆虫的隔离时间. 这项研究确定了B. mori arylalkylamine N-acetyltransferase (BmaaNAT) 作为关键的效应因子,将时钟信号与隔离连接起来,揭示了季节性适应的新机制.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- 循环时钟基因影响昆虫的隔离,但下游的效应者是未知的.
- 在Bombyx mori中,隔膜期取决于母亲发育期间的温度和光周期线索.
- 无时无刻的 (Tim) 是一个核心的昼夜钟基因,对隔离器诱导至关重要.
研究的目的:
- 为了识别Bombyx mori diapause中昼夜钟基因的下游效应分子.
- 为了研究B. mori arylalkylamine N-乙转移酶 (BmaaNAT) 在隔离调节中的作用.
- 阐明将昼夜时钟输出与隔离时间联系起来的分子机制.
主要方法:
- 野生类型和无时 knockout (Tim-/-) 丝虫胚胎的无标签定量 (LFQ) 蛋白质组学.
- 双化酶试验证实了BmaaNAT的转录调节.
- 在丝虫中使用BmaaNAT淘汰和过度表达的功能验证.
主要成果:
- 在用于隔膜期的胚胎中,BmaaNAT显著下调.
- CLOCK/CYCLE (CLK/CYC) 异构体直接激活了BmaaNAT的转录.
- BmaaNAT操纵影响了幼虫的持续时间和隔离期的发生,过度表达部分拯救了Tim-/-突变.
结论:
- BmaaNAT是一个关键的效应器,将昼夜时钟信号与隔离连接起来.
- 这项研究揭示了昆虫季节性适应的新型分子机制.
- BmaaNAT在整合环境线索中起着关键作用,用于对隔距的确定.
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