相关实验视频

Updated: May 12, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

用RIP-seq和RIP-qPCR评估哺乳动物细胞中的RNA-蛋白关联的协议

Jackson B Trotman1, Shuang Li1, Quinn E Eberhard2

  • 1Department of Pharmacology, RNA Discovery Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

STAR protocols
|December 27, 2025
PubMed
概括

本研究详细介绍了一项用于测量哺乳动物细胞中的RNA-蛋白相互作用的协议,使用RNA免疫沉,然后进行测序 (RIP-seq) 和定量PCR (RIP-qPCR). 该方法提供了一种标准化的方法来分析这些关键的分子相互作用.

关键词:
抗体是对抗体的一种.生物信息学是一种生物信息学.在 ChIP-seqq.基因表达 基因表达在RNA-seqqq.序列分析是指进行序列分析.序列化是指测序的使用.

更多相关视频

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...