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Updated: Jun 30, 2026

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使用SeEN-seq和冷EM结构确定进行转录因子-核细胞相互作用的综合分析协议

Wataru Kobayashi¹, Alicia K Michael², Siwat Ruangroengkulrith¹

¹Department of Totipotency, Max Planck Institute of Biochemistry (MPIB), Munich, Germany.

|December 27, 2025

概括

先进的转录因子 (TFs) 可以在核体内结合DNA,影响细胞分化. 我们开发了核酶体测序的选择性参与 (SeEN-seq) 来映射这些核酶体上的TF结合位点.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

开拓性转录因子 (TFs) 对于在细胞分化和重编程等过程中启动基因表达变化至关重要.
这些TF可以访问DNA图案,即使它们被包装在核细胞体内.

研究的目的:

提出一种新的协议,选择了核细胞序列测序 (SeEN-seq) 的参与,用于系统地识别核细胞上潜在的转录因子结合位.
为了使先驱TF-核酶体复合体的结构分析.

主要方法:

该协议涉及核细胞库组装.
执行SeEN-seq试验的执行.
用单粒子分析为冷电子显微镜 (cryo-EM) 准备样本.

主要成果:

通过SeEN-seq协议,可以更容易地识别核酶体上的TF结合位.
它可以制备适合结构确定的一致的先驱TF-核酶体复合体.

结论:

SeEN-seq是一种有价值的方法,用于研究与核细胞的先驱TF相互作用.
该协议有助于理解TF介导的基因调节在染色体内的结构基础.

关键词:

高通量选的高通量选显微镜的使用方法结构生物学是结构生物学.

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