综合性血清CircRNA分析确定CircRNA_103372作为脊椎间盘退行症的新型诊断生物标志物
Shuai Li1, Qinghua Yang1, Longao Huang1
1Department of Spine Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
Biochemical and biophysical research communications
|December 27, 2025
概括
在椎间盘退化 (IDD) 患者中,血清circRNA_103372的下调. 这一发现表明circRNA_103372可能成为IDD诊断的新型非侵入性生物标志物.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 椎间盘退化 (IDD) 是一个普遍的疾病.
- 循环RNAs (circRNAs) 涉及到各种生物过程.
- 确定IDD可靠的生物标志物对于早期诊断和治疗至关重要.
研究的目的:
- 为了研究IDD患者血清中circRNAs的差异表达模式.
- 为了确定IDD的潜在circRNA生物标志物.
主要方法:
- 来自IDD患者的血清样本使用circRNA微阵列进行分析.
- 进行了生物信息学分析,包括基因本体学,Venn和蛋白质-蛋白质相互作用 (PPI) 网络.
- 使用qRT-PCR和免疫组织化学 (IHC) 验证关键的分子相互作用 (circRNA_103372-miR-205-5p-CREB1).
主要成果:
- 总共有401个差异表达的circRNAs被确定.
- circRNA_103372在IDD患者的血清和细胞核 (NP) 组织中显著下调.
- 构建了一个circRNA_103372-miR-205-5p-CREB1相互作用网络,验证显示circRNA_103372和CREB1的下调和 degenerated NP细胞中的miR-205-5p的上调.
结论:
- 血清circRNA_103372在IDD患者中显著下调.
- circRNA_103372显示出作为IDD新型,非侵入性循环生物标志物的潜力.
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