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基于网络的多组学和转基因验证表明,OsPHR3在种子发育过程中调节酸碳代谢权衡

Lekha Pazhamala¹, Mandavi Pandey¹, Priyanka Deveshwar¹

¹National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, Delhi, 110067, India.

Plant physiology and biochemistry : PPB

|December 27, 2025

概括

米粒的发育涉及复杂的酸盐 (Pi) 分配,对产量至关重要,但受到植物酸 (PA) 降低营养的阻碍. 这项研究确定了OsPHR3作为平衡Pi,糖和PA的关键调节器,为可持续农业提供了目标.

科学领域:

植物生物学植物生物学
农业科学农业科学
分子生物学分子生物学

背景情况:

在谷物填充过程中酸盐 (Pi) 的分配对作物产量至关重要,影响宏分子合成和能量代谢.
植物酸 (PA) 积累通过化必需矿物质,降低了谷物的营养质量.
在种子中整合Pi运输,碳水化合物代谢和PA生物合成的分子机制尚未完全理解.

研究的目的:

调查种子发育期间在中的特定阶段的酸盐调节网络.
阐明酸盐缩反应3 (OsPHR3) 在协调Pi,碳水化合物和PA代谢中的作用.
确定参与营养储存和应激耐受性的关键基因.

主要方法:

转录基因,蛋白质基因和代谢基因数据的整合.
时间表达概况和基因共表达网络分析.
在CRISPR/Cas9基因编辑中生成osphr3淘汰线.

主要成果:

OsPHR3充当调节Pi,糖,粉和植物酸盐平衡的中心枢纽.
网络分析确定了126个与营养储存和耐压力相关的基因,包括OsMIPS1和OsSSIII.
OsPHR3淘汰突变体显示种子粉,PA和含量降低,粉颗粒形态变化和PA的19.46-22.50%降低.

结论:

OsPHR3是水种子发育过程中特定阶段酸盐-碳分配的关键调节者.
针对OsPHR3提供了提高作物产量,降低植物酸含量和提高使用效率的潜力.
这些发现通过优化作物中的营养分区,有助于农业的可持续性.

关键词:

谷物填充谷物填充多种组合的多种组合.在 OsPHR3 里,酸盐是一种酸盐.植物酸盐植物酸盐大米大米大米大米大米种子的种子开发.

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