甲基化物敏感的VviDOF3.4-VviLBD36模块调节葡萄藤中的偶然根形成
Rui Yu1, Pengwei Jing1, Jiayin Niu1
1College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China; Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang, 471023, Henan Province, China.
Plant physiology and biochemistry : PPB
|December 27, 2025
概括
甲化物 (CCC) 增强了葡萄树的偶然根 (AR) 形成. 这种效应是由VviDOF3.4转录因子激活VviLBD36的介导,促进AR的发展.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 偶然的根 (AR) 形成对于葡萄藤无性繁殖至关重要.
- 甲基化物 (CCC) 是一种吉伯雷林抗剂,调节植物生长,但其在AR形成中的作用尚未得到充分研究.
研究的目的:
- 调查CCC对"普森无种"葡萄树中AR形成的影响.
- 为了确定参与CCC介导的AR形成的关键分子因素.
主要方法:
- 对葡萄藤进行CCC处理.
- 转录组对AR的测序
- 基因表达分析 (阿拉比多普西斯中的过度表达)
- 酵母单杂交和双化酶测定方法
主要成果:
- 治疗CCC显著促进了葡萄树的AR形成.
- 鉴定出VviLBD36是一种由CCC上调的关键基因,促进AR和侧根 (LR) 发育.
- 发现VviDOF3.4直接结合并激活VviLBD36促进体,也促进了LR的发展.
结论:
- VviDOF3.4-VviLBD36模块在葡萄树中CCC诱导的AR形成中发挥着至关重要的作用.
- 这项研究为了解CCC对葡萄树根发育的影响提供了分子基础.
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