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在BioFire® FilmArray®分析中发现血流感染的真菌假阳性

Osamu Imataki¹, Ryoko Masumoto², Masahiro Hamano²

¹Department of Laboratory Medicine, Kawasaki Medical School, General Medical Center, 2-6-1 Nakasange, Kita-district, Okayama city, Okayama 700-8505, Japan; Department of Laboratory Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki city, Okayama 701-0192, Japan.

Journal of microbiological methods

|December 27, 2025

概括

在使用BioFire FilmArray®血液培养识别面板2 (BCID2) 系统检测真菌时,观察到错误阳性,特别是Candida tropicalis. 这些结果突出了基于PCR的真菌鉴定的局限性,强调需要在临床背景下仔细解释.

科学领域:

临床微生物学临床微生物学
分子诊断学分子诊断学
传染性疾病传染性疾病

背景情况:

生物火电影Array®系统是一种用于病原体检测的自动化多重PCR工具.
假阳性可能来自交叉反应,污染或试验特异性问题.
这项研究的重点是FilmArray® BCID2面板的假阳性真菌结果.

研究的目的:

使用FilmArray® BCID2系统调查假阳性真菌结果的发生率和特征.
为了识别与虚假阳性相关的特定的Candida物种.
探索这些假阳性发现的潜在原因.

主要方法:

在12个月内收集了疑似血流感染的潜在病例.
使用标准培养方法和质谱法 (VITEK MS PRIME) 来识别真菌.
将结果与BioFire FilmArray® BCID2面板进行比较.

主要成果:

评估了454个血液培养样本,确定了28例真菌病 (6.2%).
观察到8个假阳性真菌结果 (1.8%),包括Candida albicans和Candida tropicalis.
四个错误的阳性Candida tropicalis结果与Candida parapsilosis的联合检测有关.

结论:

在使用FilmArray® BCID2系统时,发现了大量的错误阳性Candida结果.
经常出现的假阳性病例涉及Candida tropicalis,通常与Candida parapsilosis一起.
潜在的原因包括不可活性的DNA片段和Candida parapsilosis/Candida tropicalis交叉反应,需要专家解释结果.

关键词:

血液培养血液培养是什么?血流感染血流感染这是错误的阳性.在FilmArray®中使用.微生物学微生物学多重分子面板的多重分子面板.酵母酵母是一种酵母.

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