来自城市湿地的多药耐药性大肠杆菌ST361的基因组表征和比较分析
Lakshmi Srijith1, Vignesh Sivakumar1, Gayathri Sivanandham1
1Department of Biotechnology, Sri Ramachandra Institute of Higher Education and Research, Porur, Tamil Nadu, India.
BMC microbiology
|December 27, 2025
概括
首例多药耐药大肠杆菌ST361在印度湿地被发现,突出显示了它们在传播抗生素耐药性的作用. 这一发现强调迫切需要更好的废水处理和抗生素使用法规,以保护"一个健康".
科学领域:
- 环境微生物学环境微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 湿地积累抗生素耐药细菌 (ARB) 和基因 (ARG) 由于人类活动,如污水和农业下水.
- 迁徙鸟类可以从受污染的湿地在全球范围内传播ARB和ARG.
- 包括ST361在内的多药耐药 (MDR) 大肠杆菌越来越多地出现在城市湖泊中,构成全球健康威胁.
研究的目的:
- 报告印度湿地首次检测到大肠杆菌ST361的情况.
- 在ST361隔离物中识别耐药性和毒性基因.
- 在全球范围内对来自临床和环境来源的ST361分离物进行比较基因组分析.
主要方法:
- 在季风季节从奈湖收集的淡水样本.
- 使用MALDI-TOF确认了大肠杆菌,并进行了抗微生物敏感性测试.
- 使用Illumina NovaSeq 6000进行全基因组测序 (WGS) 来进行基因组表征.
主要成果:
- 大肠杆菌分离物 (KOTW5) 呈现出对安培素,四环素和胆固醇素的耐药性.
- KOTW5被确定为ST361,具有耐药性基因 (pmr, TolC, acr, sul2) 和50个毒性基因 (fim, esp, gsp).
- 对比基因组学揭示了KOTW5与坦桑尼亚和德国的临床大肠杆菌分离物之间的密切关系,表明人类感染的潜力.
结论:
- 湿地可以作为MDR大肠杆菌持续和传播的热点.
- 湖泊中ST361的存在强调了人类,动物和环境健康 (One Health) 的相互联系.
- 包括先进的废水处理和更严格的抗生素法规在内的全面战略对于打击水生环境中的抗菌素耐药性 (AMR) 至关重要.
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