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通过超声波和微气泡触发的乳腺癌细胞中的Piezo1介导的细胞亡

Nina Qu¹, Menglu Bi², Qingkai Meng³

¹Department of Ultrasound Medicine, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China. ninebaby1006@126.com.

Cellular and molecular biology (Noisy-le-Grand, France)

|December 28, 2025

概括

超声波微泡 (USMB) 治疗和Piezo1激动剂 (Yoda1) 通过激活ER压力和线粒体通路,促进乳腺癌细胞亡. 皮埃佐1抑制剂 (GsMTx4) 逆转了这些影响,突出了皮埃佐1的作用.

科学领域:

细胞生物学细胞生物学
在瘤学瘤学.
生物物理学的生物物理.

背景情况:

皮埃佐1离子通道是涉及细胞过程的机械敏感通道.
亡的失调有助于乳腺癌的进展.
准Piezo1为乳腺癌提供了潜在的治疗策略.

研究的目的:

研究Piezo1在人类乳腺癌细胞 (MCF-7) 亡中的作用.
评估Piezo1调节使用Yoda1 (激动剂) 和GsMTx4 (抑制剂) 对亡的作用.
评估超声波微泡 (USMB) 治疗对Piezo1-介导的亡的影响.

主要方法:

西方涂抹测量乳腺癌细胞系中的Piezo1蛋白表达.
流细胞计测量以测量亡速率.
评估与亡相关的蛋白质,ER压力标志物和线粒体通路蛋白质.
使用光探针测量线粒体膜潜力和细胞内离子 (Ca2+) 度.

主要成果:

MCF-7细胞表现出Piezo1表达的最高水平.
约达1和USMB显著增加了细胞亡,ER压力和线粒体通路激活.
GsMTx4抑制了亡并逆转了USMB诱导的影响.
治疗USMB导致线粒体膜潜能降低,细胞内Ca2+增加.

结论:

在乳腺癌细胞中,USMB治疗通过Piezo1激活ER压力.
USMB诱导线粒体功能障碍,并提高细胞内Ca2 +,促进细胞亡.
调节Piezo1活动为乳腺癌治疗提供了潜在的治疗途径.

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