通过一种新型细菌-酵母菌微生物组进行面向的酸盐生产:批量验证,关键电子供体识别和长期验证
Yuan Li1, Yueji Chen1, Zonghai Du1
1Shanghai University of Engineering Science, School of Chemistry and Chemical Engineering, Shanghai, China.
Bioresource technology
|December 28, 2025
概括
添加Saccharomyces cerevisiae (SC) 增强了从有机废物中生产的酸盐. 最佳的SC度 (2g/L) 提高了酸盐合成的2.72倍,改善了循环经济过程.
科学领域:
- 生物技术是生物技术.
- 循环经济是一个循环经济.
- 废弃物价值化 废弃物价值化 废弃物价值化
背景情况:
- 传统的酸盐发酵主要集中在碳水化合物和蛋白质上.
- 酸盐合成的链延长 (CE) 需要优化.
- 有机废物回收符合循环经济原则.
研究的目的:
- 评估Saccharomyces cerevisiae (SC) 对乙醇生产的影响,以及对于丁酸盐合成的CE.
- 确定最佳的SC度,以提高丁酸盐的生产.
- 研究酵母-细菌协同作用,以有效地利用废物.
主要方法:
- 对SC度 (1-8 g/L) 的系统评估.
- 代谢途径和元基因组分析 (16S rRNA).
- 用蒸酵母酵母注射剂进行长期试验.
主要成果:
- 最佳的SC剂量为2g/L,产生15.41g的COD/L丁酸盐 (2.72x高于对照).
- SC增强了基质降解 (>90%) 和 in situ 乙醇利用.
- 顺序内射注射丰富了克洛斯特里物种,并促进了关键的代谢基因.
结论:
- 酵母菌-细菌协同作用对于增强有机废物中的酸盐合成至关重要.
- 添加SC可以稳定生产并提高效率.
- 提供了一个循环经济框架内的食品废弃物利用战略.
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