在盐应激下,Populus euphratica PeNAC029抑制了脂酶D和酸信号传递
Ziyan Zhao1, Rui Shi1, Ying Zhang1
1Key Laboratory of Forest and Flower Genetics and Breeding of Ministry of Education, College of Biological Science and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Plant physiology and biochemistry : PPB
|December 28, 2025
概括
转录因子PeNAC029抑制了脂酶D (PLD) 基因,通过破坏和活性氧物种的恒温,阻碍了植物的盐耐受性. 降低PeNAC029的调节有助于木抵抗盐.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 转录条例 转录条例 转录条例
背景情况:
- 在盐应激下,Populus euphratica 脂酶 Dδ (PePLDδ) 对于维持 (Na+) 和活性氧物种 (ROS) 恒温至关重要.
- 了解PLDs的转录调节是改善植物盐耐受性的关键.
研究的目的:
- 为了确定在盐应力下调节PePLDδ表达的转录因子.
- 阐明PeNAC029在植物盐耐受性中的作用及其对PLD基因的调节.
主要方法:
- 通过DNA拉下测定和质谱测试,确定PeNAC029是PePLDδ促进体结合蛋白.
- 在盐应激下对Arabidopsis和P. euphratica进行基因表达分析.
- 对PLD活性和酸 (PA) 水平的研究.
主要成果:
- 含有类似NARD的域的PeNAC029,与PLD促进体中的ACGTcis元素结合,抑制PLD基因表达 (例如AtPLDδ,PePLDα3,PePLDβ2).
- 过度表达PeNAC029通过抑制Na+/H+抗载体和抗氧化酶基因,降低了盐分耐受性,降低了PLD活性,降低了PA水平,并损害了Na+和ROS稳态.
- 在长时间的盐应激下,PeNAC029的表达下降,这表明耐盐树中存在一种保护机制.
结论:
- PeNAC029通过抑制PLD介导的信号通路,作为植物盐耐受性的负调节剂.
- 在盐应激下PeNAC029的下调是Populus中盐耐受性的关键适应性反应.
- 一个涉及PeNAC029和PLDs的转录调节网络被提议用于盐应激反应.
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