Clostridium tyrobutyricum 的代谢工程用于六酸和六醇生产
Shangjun Wang1, Jie Zhang1, Pixiang Wang1
1Department of Biosystems Engineering, Auburn University, Auburn 36849 AL, USA.
Bioresource technology
|December 28, 2025
概括
工程微生物现在可以生产有价值的六碳化学物质,如六酸和六醇. 这种可持续的生物合成方法实现了创纪录的产量,为以石油为基础的生产提供了替代品.
科学领域:
- 合成生物学和代谢工程 合成生物学和代谢工程
- 微生物生物技术 微生物生物技术
- 生物化学工程 生物化学工程
背景情况:
- n-hexanoic酸和n-hexanol是重要的工业化学品.
- 目前的生产依赖于石油,激励可持续的生物合成.
- 微生物生产受到毒性和途径低效的限制.
研究的目的:
- 为了设计Clostridium tyrobutyricum以有效生产C6酸和酒精.
- 为了利用CoA依赖的反向β-氧化途径进行碳链延伸.
主要方法:
- 系统评估关键酶,以延长碳链从丁-CoA.
- 工程类Clostridium tyrobutyricum通过共同表达特定的酶,包括硫酶 (ThlCPB6).
- 修改原生CoA转移酶 (Cat1) 与CPB6的同类物来增强六酸标位.
主要成果:
- 鉴定了鲁米诺科卡ceae 细菌 CPB6 酶 (ThlCPB6) 作为 C6--CoA 形成的必要条件.
- 实现了 de novo hexanoate 合成,标位高达 4.4 g/L,这是工程菌株中报告的最高值.
- 在一个醇生产的工程菌株中生产高达91毫克/升的六醇.
结论:
- 酸 (Clostridium tyrobutyricum) 是一种强大的底盘,可以通过逆β氧化产生中链酸和酒精.
- 证明了一种可行的微生物平台,用于增值化学品的可持续生物合成.
- 开辟了利用红纤维素生物质用于化学生产的途径.
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