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在老鼠卵子细胞的排卵后衰老模型中,内分泌网膜应激和线粒体活动之间的功能相互作用

Fumihiro Nakamura¹, Isao Takehara², Saki Hine¹

¹Department of Obstetrics and Gynecology, Faculty of Medicine, Yamagata University, 2-2-2, Iida-Nishi, Yamagata, 990-9585, Japan.

Journal of assisted reproduction and genetics

|December 28, 2025

概括

针对内质网膜或线粒体,在一个排卵后衰老模型中,单独改善了胚胎发育. 这些器官特异性调制增强了胚胎细胞的形成,并减少了老化的卵细胞中的细胞死亡.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
线粒体和ER功能.

背景情况:

母亲的衰老会影响卵细胞质量和胚胎发育.
细胞内膜网膜 (ER) 和线粒体的相互作用对细胞平衡至关重要.
功能障碍的ER-线粒体轴有助于与年龄相关的卵细胞衰退.

研究的目的:

为了研究是否调节ER-线粒体轴可以增强胚胎发育在后排卵衰老 (POA) 鼠标模型.
评估器官特异性干预对卵细胞质量和胚胎细胞形成的影响.

主要方法:

卵子从新鲜和经卵期老化 (POA) 后的小鼠模型中收集.
新鲜卵子被用ER压力 (thapsigargin) 或线粒体抑制剂 (CCCP) 治疗,以评估压力传播.
POA卵子被用盐 (ER调节器),5 - 氨基风氨酸 (5-ALA,线粒体调节器) 或组合治疗.
胚胎发育是通过胚胎细胞形成率和亡来评估的.

主要成果:

在新鲜卵细胞中观察到ER和线粒体之间的双向压力信号.
在POA卵细胞中,沙路布林减少了ER压力标志物,5-ALA降低了线粒体超氧化物水平.
单一药物和组合疗法都没有对ER或线粒体标记物产生添加效应.
所有治疗组,包括单剂治疗,在POA卵细胞中显著改善了芽细胞形成和减少了亡.

结论:

针对ER或线粒体独立地为POA卵细胞的胚胎发育提供了适度的好处.
在新鲜卵细胞中观察到的ER-线粒体合在POA模型中显得减弱.
这些发现为进一步研究母亲衰老干预提供了基础,并指出在生理氧气条件下,结果可能有所不同.

关键词:

艺术艺术艺术艺术胚胎囊形成的过程.压力ERER压力ERER压力线粒体功能障碍线粒体功能障碍卵细胞衰老卵细胞衰老

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