印度城市污水微生物群中的抗生素污染和抗菌素耐药性动态
Deepjyoti Paul1, Daizee Talukdar1, Ramani Shyam Kapuganti1
1Microbial Research Centre, BRIC-Translational Health Science and Technology Institute (THSTI), Faridabad, India.
Nature communications
|December 28, 2025
概括
污水作为抗微生物耐药性基因 (ARG) 的主要储存库,促进了耐药细菌的传播. 一个新的测量棒测定有助于跟踪在低资源设置中的阻力.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 抗微生物耐药性 (AMR) 是一个全球性的健康威胁,由抗微生物耐药性基因 (ARG) 的传播驱动.
- 在密集的微生物环境中,如污水中,亚致杀伤性抗生素度可以促进ARG的获取和传播.
研究的目的:
- 调查污水作为ARG和多药耐药 (MDR) 细菌病原体的储存库.
- 评估印度污水样本中抗生素度,微生物多样性和ARG丰富度之间的相关性.
- 开发一种用于监测目的的污水中ARG的快速检测方法.
主要方法:
- 分析了来自印度六个州的381个污水样本,以检测抗生素度,微生物多样性 (ASV) 和ARG丰富度.
- 大基因组和分离基因组测序 (n=220 + 305) 来识别ARG和相关的移动遗传元素 (MGE).
- 对MDR细菌分离物 (n=7166) 的比较核心基因组分析,以比较污水衍生的菌株与临床病原体.
主要成果:
- 检测到7种药物类别的抗生素和2000多种细菌ASVs.
- 确定了82个与80个MGE相关的ARG,主要在MDR病原体中.
- 在污水衍生的MDR菌株和临床病原体之间发现了显著的遗传相似性,证实了污水是ARG储存库.
结论:
- 污水是ARG的关键储存库,促进了AMR病原体的进化和传播.
- 污水中的基因交换在社区和医疗保健环境中都有助于AMR.
- 开发的一种基于测量棒的测定方法为资源较少的地区的污水监测提供了一个可行的工具.
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