基于过的快速ECM-水凝消除使肠道器官的强大的MALDI MSI成为可能
Fangyuan Zhang1,2, Xinxin Shen2, Jie Yuan2
1School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310058, China.
Analytical chemistry
|December 29, 2025
概括
我们开发了一种快速过方法 (免费) 来快速从肠道有机体中去除细胞外基质液,以进行代谢分析. 这种技术提高了代谢物覆盖率,并保留了有机体结构,改善了质谱成像数据的质量.
科学领域:
- 生物医学工程 生物医学工程
- 代谢学 代谢学 代谢学
- 胃肠病学 胃肠病学
背景情况:
- 矩阵辅助激光脱/电离质谱成像 (MALDI MSI) 对肠道有机体的二维代谢分析至关重要.
- 目前用于细胞外基质 (ECM) 水凝去除的方法耗时,可能会损害有机体和代谢物.
研究的目的:
- 开发一种有效的方法来从肠道器官中去除ECM水凝,以改进MALDI MSI分析.
- 评估新方法对有机体完整性,代谢稳定性和数据质量的影响.
主要方法:
- 建立了基于过的快速ECM-水凝清除 (FREE) 方法,使用70微米的过器.
- 在时间,有机体完整性,细胞活力,代谢稳定性和数据质量方面与传统方法相比,FREE.
- 将FREE工作流应用于LPS诱导的炎症性肠道有机体模型.
主要成果:
- 免费方法将样本准备时间从75分钟缩短到25分钟.
- 自由保存有机体结构,细胞活力和代谢稳定性,同时提高光谱质量.
- 与传统方法相比,其代谢物覆盖率增加了26.6%,信号强度提高.
- 在炎症模型中观察到空间代谢重塑和改变脂质分布 (酸和酸乙醇胺),与极性丧失有关.
结论:
- 免费方法是一种有效的预处理策略,用于MALDI MSI的肠道有机体.
- 这种方法提高了数据质量,并使得与炎症相关的代谢重塑及其与细胞过程 (如亡) 的联系的研究成为可能.
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