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过度表达miR-516a-5p促进侵蚀性口腔液状平原:基于生物信息学分析的体外研究

Yan Chen¹, Chen-Xi Li^2,3, Rui Xue⁴

¹Division of Pediatric Dentistry and Preventive Dentistry, School/Hospital of Stomatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

Clinical and experimental dental research

|December 29, 2025

概括

在侵蚀性口平中过度表达的MicroRNA-516a-5p可能会改变炎症性细胞因子,并通过向MAPK11来促进纤维细胞的增殖和迁移. 这一发现揭示了OLP的病原性.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

侵蚀性口腔平坦 (EOLP) 是一种具有复杂分子基础的炎症状况.
微RNAs (miRNAs) 是基因表达的关键调节者,与各种疾病有关.

研究的目的:

在EOLP中识别差异表达的miRNA.
研究miR-516a-5p在人类口腔粘膜纤维细胞 (OMF) 中的功能作用.

主要方法:

从EOLP组织和健康对照中测序miRNA表达特征的高通量测序.
生物信息学分析以确定丰富的途径和向基因.
在体外实验中涉及OMFs中的miR-516a-5p过度表达的实验.

主要成果:

82个miRNA在EOLP中被差异表达,主要与T辅助细胞分化和MAPK信号通路有关.
miR-516a-5p过度表达显著降低了OMF中的MAPK11mRNA水平.
miR-516a-5p改变了炎症性细胞因子 (增加了TNF-α,IFN-γ;减少了IL-4,IL-6,IL-10,IL-13) 和增强了OMF的增殖和迁移.

结论:

在EOLP中的miR-516a-5p过度表达有助于T助手1/2细胞相关的炎症性细胞因子失衡.
通过miR-516a-5p针对MAPK11mRNA促进OMF的增殖和迁移,可能会影响EOLP的进展.

关键词:

帮助T细胞的帮助T细胞.细胞因子细胞因子侵蚀性口腔平坦的平坦.人类口腔粘膜纤维细胞.在 miR-516a-5p 中.

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