相关实验视频

Updated: Jan 7, 2026

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Published on: October 27, 2019

以参考为指导的基因组组合草稿的苗条行走的鱼,Prophagorus nieuhofii

Imron Imron¹, Fajar Anggraeni¹, Rahmat Hidayat¹

¹Research Center for Fishery, National Research and Innovation Agency Republic of Indonesia, Cibinong, West Java, 16915, Indonesia.

|December 29, 2025

概括

为了帮助可持续的渔业管理,精细行走的鱼 (Prophagorus nieuhofii) 的基因组被测序. 这种遗传资源支持东南亚这一重要的粮食安全物种的保护工作.

科学领域:

水生基因组学在水生基因组学.
渔业科学渔业科学
保护生物学保护生物学

背景情况:

瘦身行走的鱼 (Prophagorus nieuhofii) 对于东南亚渔业的粮食安全至关重要.
目前的保护状况是最不关心,但过度捕捞和息地丧失等威胁带来风险.

研究的目的:

为了测序,组装和注释普罗法哥拉斯 (Prophagorus nieuhofii) 的整个基因组.
为可持续的渔业管理和水产养殖提供基因组资源.
支持对这一经济重要物种的保护战略.

主要方法:

使用27GB的数据进行全基因组测序.
基因组组装导致1.1Gb与5,790支架.
重复注释和结构注释以识别基因和转录.

主要成果:

一个高度连续和完整的基因组组件 (N50: 33.7 Mb,BUSCO: 98.8%).
识别重复元素 (48.17%),包括DNA转位子和反复元素.
30,099个蛋白质编码基因和37,734个转录的注释,揭示了广泛的替代拼接.

结论:

这种高质量的基因组组件为Prophagorus nieuhofii提供了基础资源.
基因组数据将促进对人口遗传学,适应和保护的研究.
这项工作支持对这一关键物种的渔业和水产养殖的可持续管理.

关键词:

普罗法哥拉斯没有办公室.整个基因组的测序.基因组注释基因组注释基因组组装组合的基因组.瘦瘦的行走的鱼

更多相关视频

Wholemount In Situ Hybridization for Astyanax Embryos

Wholemount In Situ Hybridization for Astyanax Embryos

Published on: March 2, 2019

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

Published on: January 20, 2018

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.