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鉴定与慢性乙型肝炎肝脏免疫炎症损伤相关的血清MicroRNAs:对非侵入性诊断的影响

Mengkui Han^1,2,3, Junchi Xu¹, Xunxun Wu¹

¹Department of Infectious Diseases, The Affiliated Infectious Diseases Hospital of Soochow University, Suzhou, Jiangsu, China.

Frontiers in immunology

|December 29, 2025

概括

新的生物标志物miR-224-5p和miR-200a-3p显示出在乙型肝炎病毒患者中诊断肝纤维化和癌症的前景. 这些循环的microRNA提供了监测疾病进展的非侵入性方法.

科学领域:

肝病学肝病学是一种肝病学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

乙型肝炎病毒 (HBV) 感染导致晚期肝脏疾病,缺乏炎症和纤维化有效的非侵入性生物标志物.
循环微RNAs (miRNAs) 是HBV相关肝病进展的关键调节剂和潜在生物标志物.

研究的目的:

在慢性乙型肝炎 (CHB) 患者中描述miRNA配置文件.
评估miRNAs与肝炎和肝炎纤维化在HBV感染中的关联.
为了确定与HBV相关的肝病病期的新型非侵入性生物标志物.

主要方法:

在使用qRT-PCR的HBV疾病连续体中,在165个个体 (发现组) 和143个 (测试组) 中进行血清miRNA分析.
监督集群,相关性分析,后勤回归和ROC/AUC评估与交叉验证和外部验证.
对miRNA目标和相关途径的生物信息预测.

主要成果:

在不同HBV疾病阶段识别出不同的miRNA表达特征.
miR-224-5p与肝硬化中的纤维化阶段有很强的相关性 (r = 0.606),并作为肝硬化强大的非侵入性生物标志物 (AUC = 0.973发现,0.906验证),表现优于APRI和FIB-4.
一个结合miR-200a-3p和AFP的模型显示了肝细胞癌 (HCC) 的高诊断准确性 (AUC>0.9).

结论:

miR-224-5p被确定为早期肝硬化检测的关键指标.
miR-200a-3p增强了非侵入性HCC诊断的AFP,为HBV疾病连续建立了双miRNA签名.
这些血清miRNA具有作为监测疾病进展和改善HBV相关肝病差异诊断的非侵入性生物标志物的潜力.

关键词:

现在,CHB已经成为了CHB.这是一种HBV HBV.在HCC中,HCC是HCC.生物标志物生物标志物肝脏纤维化肝脏纤维化这就是miRNAs.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...