相关实验视频

Updated: Jan 7, 2026

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

利奇病原体 (Phytophthora litchii) 的完整线粒体基因组

Jinhua Sun¹, Min Li¹, Gengxin Chen¹

¹Key Laboratory of Integrated Pest Management on Tropical Crops, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Haikou, China.

Microbiology resource announcements

|December 29, 2025

概括

测序了Phytophthora litchii株 hk-1 的线粒体基因组. 这项研究详细介绍了它的遗传构成,包括基因和AT含量.

科学领域:

* 基因组学是一个学科.
* 分子生物学 * 分子生物学
* 菌群学 * 菌群学

背景情况:

*Phytophthora litchii是一种影响litchi作物的植物病原体.
* 了解其遗传构成对于疾病管理至关重要.
*线粒体基因组为进化关系和代谢功能提供了洞察力.

研究的目的:

* 组装和注释 Phytophthora litchii 菌株 hk-1 的完整线粒体基因组.
* 描述基因组特征,包括大小,基因组成和基因含量.
*为Phytophthora litchii研究提供一个基础的基因组资源.

主要方法:

*从 Phytophthora litchii 菌株 hk-1 中提取DNA.
* 全基因组测序和组装.
* 对线粒体基因组的基因预测和注释.

主要成果:

*成功组装了Phytophthora litchii hk-1菌株的完整线粒体基因组.
* 基因组是圆形的,测量38,126个基对 (bp).
* 该基因组富含AT,高AT含量为78.26%,含有2个核糖体RNA基因,25个转移RNA基因和41个蛋白质编码基因.

结论:

*特征化的线粒体基因组为Phytophthora litchii提供了宝贵的遗传资源.
* 基因组数据可以帮助我们了解这种虫虫的进化和病原性.
* 进一步的研究可以利用这个基因组进行比较基因组学和遗传学分析.

关键词:

植物 (Phytophthora litchii) 是一个有机植物.一个小小的小小的小小的小小.线粒体基因组的基因组

更多相关视频

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

相关概念视频

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Export of Mitochondrial and Chloroplast Genes

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...

Animal Mitochondrial Genetics

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...